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COCOON



Center for Optical Characterization
Of Organic and Natural materials Center for Optical Characterization Of Organic and Natural materials

A multiscale, correlated imaging and spectroscopy suite to study biomaterials
from the macro to the nanoscale.

About COCOON

COCOON is a next-generation characterization suite designed for researchers working at the intersection of biology, materials science, and optics.

Built around a scanning electron microscope, COCOON integrates Raman spectroscopy, elemental analysis, and cryogenic sample preparation into a single unified platform.

This environment enables something rare: direct, real-time correlation of composition, structure, and function across orders of magnitude in scale, all within one workflow, at both room temperature and cryogenic conditions.

Researchers can move seamlessly from whole-sample imaging to nanoscale structural mapping, from molecular identity to elemental distribution, and from dynamic behavior to high-resolution visualization. This combination allows COCOON to reveal relationships in natural and engineered materials that would otherwise remain hidden.

COCOON is a Tufts University Core Facility, supported by the Office of Naval Research and the W. M. Keck Foundation.

Multiscale Structure–Function Characterization

COCOON enables a level of correlated multimodal analysis that is uncommon even in advanced microscopy centers. By tightly integrating optical, spectroscopic, elemental, and electron-based techniques, the platform allows researchers to:

  • Connect micro- and nanoscale architectures with chemical composition
  • Visualize fragile biological and soft materials without distortion
  • Build multiscale, quantitative maps of morphology, assembly, and molecular organization
  • Capture how natural systems manage light, forces, and interfaces in real time
    Core Capabilities:
  • Cryogenic Scanning Electron Microscopy (Cryo-SEM)
    Preserves the true morphology and assembly of biological and soft materials, minimizing dehydration or collapse.
  • Energy-Dispersive X-ray Spectroscopy (EDX)
    Provides spatially resolved elemental composition, revealing gradients, inclusions, and material heterogeneity.
  • Raman Spectroscopy (RISE)
    Identifies molecular species and maps chemical organization directly on the SEM stage for one-to-one correlation.

Together, these tools deliver a comprehensive, multiscale picture of how organic, biological, and natural materials are built and how they function.

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Instrumentation

COCOON houses a tightly integrated set of instruments optimized for cross-correlated analysis. This integrated suite ensures that samples can be tracked across instruments without losing spatial context, enabling true correlative microscopy.

    Core System
  • Zeiss Sigma 300 FE SEM with GEMINI column equipped with:
    • Oxford Instruments AztechLive standard EDX System
    • Oxford Instruments fast confocal Raman RISE module
    • Quorum turbo-pumped cryo system with preparation chamber
    Supporting Platforms
  • Quorum sputter coater and evaporator
  • ZEISS Axio Imager.Z2m with LSM900 confocal scanner
  • ZEISS Stemi stereomicroscope
  • Bruker Innova atomic force microscope
  • CRI Nuance multispectral cameras

Access & Rates

COCOON operates in assisted-use mode. All imaging sessions are carried out by trained staff who operates the microscope for you to ensure the highest quality data and optimal instrument performance.

Because sample preparation is critical to imaging outcomes, we encourage researchers to consult with us before preparing or submitting samples. Even when limited by preparation constraints, our team will obtain the best possible images and spectra.

Biosafety Level 2 (BSL-2) materials are not permitted in this facility.

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Contacts

For scheduling and inquiries on scientific collaborations, please contact:

Research Prof. Giulia Guidetti, giulia.guidetti@tufts.edu
Silvia Betti, silvia.betti@tufts.edu
Prof. Fiorenzo Omenetto, fiorenzo.omenetto@tufts.edu

Location
Silklab, Suite 4875
200 Boston Avenue
Medford, MA 02155